Some examinations on antigenic and other properties of α-casein tryptic hydrolysates

II. Inhibition reactions used in the detection and measurement of antigenicities of α-casein tryptic hydrolysates

Abstract

In this experiment immunological methods for the detection of antigenicities of α-casein and F-S fraction (soluble fraction at pH4.6 which was prepared from the α-casein tryptic hydrolysate) have been experimentally discussed.
The results obtained are summarized as follows:
1) In double diffusion and immunoelectrophoretic analyses, two precipitin lines were found in the α-casein-anti-α-casein serum system, but no line was found in the F-S fraction-anti-α-casein serum system. These results indicated that double diffusion and immunoelectrophoretic analyses were incapable methods for detecting antigenicities of low molecular weight substances such as F-S fraction.
2) Antigenicities of α-casein and F-S fraction were obviously detected by haemagglutination-inhibition and passive cutaneous anaphylaxis-inhibition reactions. The antigenicity of F-S fraction was also detected by precipitation-inhibition reaction and the activity of the antigenicitywas found to be relatively high.
As the result of this experiment, it was found that these methods were very useful for detecting antigenicities of milk components and their enzymatic hydrolysates.