Abstract
The present study was undertaken to provide some basic aspects of the intracellular protease of Leuconostoc citrovorum IAM 1087. Leu. citrovarum producted intracellular protease optimally at 30C and production of intracellular protease reached a maximum in 24hr. The optimum pH of the intracellular protease which was prepared from the cells grown at 30C for 24hr by sonication and ammonium sulfate precipitation was 7.5 in phosphate buffer. The optimum temperature was 45C, and the inactivation of the protease was observed to begin already even at 40C. The protease hydrolyzed more actively αs-casein than the other casein fractions.
