豚卵胞の顆粒層細胞の走査電子顕微鏡的研究

宮本 元; 古林 亮介; 谷井 隆夫; 石橋 武彦

doi:10.2508/chikusan.53.831

Scanning Electron Microscopy of Granulosa Cells in the Porcine Ovarian Follicle

Hajime MIYAMOTO, Ryosuke FURUBAYASHI, Takao TANII, Takehiko ISHIBASHI

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JOURNAL FREE ACCESS

1982 Volume 53 Issue 12 Pages 831-837

DOI https://doi.org/10.2508/chikusan.53.831

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Published: December 25, 1982 Received: July 13, 1982 Available on J-STAGE: March 10, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: March 10, 2008 Reason for correction: - Correction: CITATION Details: Wrong : 1) SHORT, R. V., Recent Progr. Hormone Res., 20: 303-340. 1965.
2) SAVARD, K., J. M. MARSH and B. F. RICE, Recent Progr. Hormone Res., 21: 285-365. 1965.
3) DORFMAN, R. I., in Handbook of Physiology. section 7. vol. 2. part 1. (GREED, R. O. and E. B. ASTDWOOD, eds.) 537-546. Williams and Wilkins Comp. Baltimore. 1973.
4) BJERSING, L., in The Ovary. 2nd ed. Vol. 1. (ZUCKERMAN, L. and B. J. WEIR, eds.) 303-391. Academic Press. New York. 1977.
5) ECKSTEIN, P., in The Ovary. 2nd ed. vol. 2. (ZUCKERMAN, L. and B. J. WEIR, eds.) 275-313. Academic Press. New York. 1977.
6) CHANNING, C. P., F. W. SCHAERF, L. D. ANDERSON and A. TSAFRIRI, Reproductive Physiology. International Review of Physiology. vol. 22 (GREEP, R. O., ed.) 117-201. University Park Press. Baltimore. 1980.
7) STEINETZ, B. G., in Handbook of Physiology. section 7. vol. 2. part 1. (GREEP, R. O. and E. B. ASTWOOD, eds.) 439-466. Williams and Wilkins Comp. Baltimore. 1973.
8) MCNATTY,K. P., in The Vertebrate Ovary. (JONES, R. E. ed.) 215-259. Plenum Press. New York. 1978.
9) CHANG, S. C. S. and R. J. RYAN, Mayo Clinic Proceedings, 51: 621-623. 1976.
10) CHANG, S. C. S., W. ANDERSON, J. C. LEWIS, R. J. RYAN and Y. H. KANG, Biol. Reprod., 16: 349-357. 1977.
11) GWATKIN, R. B. L. and H. W. CARTER, in Scanning Electron Microscopic Atlas of Mammalian Reproduction (HAFEZ, E. S. E. ed.) 270-279. Igaku Shoin Ltd. Tokyo. 1975.
12) HANSEL, W., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 223-265. Academic Press. New York. 1959.
13) BODA, J. M., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 335-357. Academic Press. New York. 1959.
14) HAFEZ, E. S. E., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed). 61-80. Lea and Febiger. Philadelphia. 1968.
15) DAY, B. N., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed.) 279-288. Lea and Febiger. Philadelphia. 1968.
16) CHANNING, C. P. and S. KAMMERMAN, Endocrinology, 92: 531-540. 1973.
17) LEE, C. Y., Endocrinology, 99: 42-48. 1976.
18) RYAN, R. J. and C. Y. LEE, Biol. Reprod., 14: 16-29. 1976.
19) PORTER, K., D. PRESCOTT and J. FRYE, J. Cell Biol., 57: 815-836. 1973.
20) BOYDE, A., R. A. WEISS and P. VESELY, Exp. Cell Res., 71: 313-324. 1972.
21) WILLOCH, M., Acta Pathol. Microbiol. Scand., 71: 35-45. 1967.
22) FOLLETT, E. A. C. and R. D. GOLDMAN, Exp. Cell Res., 59: 124-136. 1970.
23) ANDREW S, P. M. and K. R. PORTER, Anat. Rec., 177: 409-426. 1973.
24) EILER, H. and A. V. NALBANDOV, Endocrinology, 100: 331-338. 1977.

Right : 1) SHORT, R. V., Recent Progr. Hormone Res., 20: 303-340. 1965.
2) SAVARD, K., J. M. MARSH and B. F. RICE, Recent Progr. Hormone Res., 21: 285-365. 1965.
3) DORFMAN, R. I., in Handbook of Physiology. section 7. vol. 2. part 1. (GREED, R. O. and E. B. ASTDWOOD, eds.) 537-546. Williams and Wilkins Comp. Baltimore. 1973.
4) BJERSING, L., in The Ovary. 2nd ed. Vol. 1. (ZUCKERMAN, L. and B. J. WEIR, eds.) 303-391. Academic Press. New York. 1977.
5) ECKSTEIN, P., in The Ovary. 2nd ed. vol. 2. (ZUCKERMAN, L. and B. J. WEIR, eds.) 275-313. Academic Press. New York. 1977.
6) CHANNING, C. P., F. W. SCHAERF, L. D. ANDERSON and A. TSAFRIRI, Reproductive Physiology. International Review of Physiology. vol. 22 (GREEP, R. O., ed.) 117-201. University Park Press. Baltimore. 1980.
7) STEINETZ, B. G., in Handbook of Physiology. section 7. vol. 2. part 1. (GREEP, R. O. and E. B. ASTWOOD, eds.) 439-466. Williams and Wilkins Comp. Baltimore. 1973.
8) MCNATTY,K. P., in The Vertebrate Ovary. (JONES, R. E. ed.) 215-259. Plenum Press. New York. 1978.
9) CHANG, S. C. S. and R. J. RYAN, Mayo Clinic Proceedings, 51: 621-623. 1976.
10) CHANG, S. C. S., W. ANDERSON, J. C. LEWIS, R. J. RYAN and Y. H. KANG, Biol. Reprod., 16: 349-357. 1977.
11) GWATKIN, R. B. L. and H. W. CARTER, in Scanning Electron Microscopic Atlas of Mammalian Reproduction (HAFEZ, E. S. E. ed.) 270-279. Igaku Shoin Ltd. Tokyo. 1975.
12) HANSEL, W., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 223-265. Academic Press. New York. 1959.
13) BODA, J. M., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 335-357. Academic Press. New York. 1959.
14) HAFEZ, E. S. E., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed). 61-80. Lea and Febiger. Philadelphia. 1968.
15) DAY, B. N., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed.) 279-288. Lea and Febiger. Philadelphia. 1968.
16) CHANNING, C. P. and S. KAMMERMAN, Endocrinology, 92: 531-540. 1973.
17) LEE, C. Y., Endocrinology, 99: 42-48. 1976.
18) RYAN, R. J. and C. Y. LEE, Biol. Reprod., 14: 16-29. 1976.
19) PORTER, K., D. PRESCOTT and J. FRYE, J. Cell Biol., 57: 815-836. 1973.
20) BOYDE, A., R. A. WEISS and P. VESELY, Exp. Cell Res., 71: 313-324. 1972.
21) WILLOCH, M., Acta Pathol. Microbiol. Scand., 71: 35-45. 1967.
22) FOLLETT, E. A. C. and R. D. GOLDMAN, Exp. Cell Res., 59: 124-136. 1970.
23) ANDREW S, P. M. and K. R. PORTER, Anat. Rec., 177: 409-426. 1973.
24) EILER, H. and A. V. NALBANDOV, Endocrinology, 100: 331-338. 1977.

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 10, 2008 Reason for correction: - Correction: CITATION Details: Wrong : 1) SHORT, R. V., Recent Progr. Hormone Res., 20: 303-340. 1965.
2) SAVARD, K., J. M. MARSH and B. F. RICE, Recent Progr. Hormone Res., 21: 285-365. 1965.
3) DORFMAN, R. I., in Handbook of Physiology. section 7. vol. 2. part 1. (GREED, R. O. and E. B. ASTDWOOD, eds.) 537-546. Williams and Wilkins Comp. Baltimore. 1973.
4) BJERSING, L., in The Ovary. 2nd ed. Vol. 1. (ZUCKERMAN, L. and B. J. WEIR, eds.) 303-391. Academic Press. New York. 1977.
5) ECKSTEIN, P., in The Ovary. 2nd ed. vol. 2. (ZUCKERMAN, L. and B. J. WEIR, eds.) 275-313. Academic Press. New York. 1977.
6) CHANNING, C. P., F. W. SCHAERF, L. D. ANDERSON and A. TSAFRIRI, Reproductive Physiology. International Review of Physiology. vol. 22 (GREEP, R. O., ed.) 117-201. University Park Press. Baltimore. 1980.
7) STEINETZ, B. G., in Handbook of Physiology. section 7. vol. 2. part 1. (GREEP, R. O. and E. B. ASTWOOD, eds.) 439-466. Williams and Wilkins Comp. Baltimore. 1973.
8) MCNATTY,K. P., in The Vertebrate Ovary. (JONES, R. E. ed.) 215-259. Plenum Press. New York. 1978.
9) CHANG, S. C. S. and R. J. RYAN, Mayo Clinic Proceedings, 51: 621-623. 1976.
10) CHANG, S. C. S., W. ANDERSON, J. C. LEWIS, R. J. RYAN and Y. H. KANG, Biol. Reprod., 16: 349-357. 1977.
11) GWATKIN, R. B. L. and H. W. CARTER, in Scanning Electron Microscopic Atlas of Mammalian Reproduction (HAFEZ, E. S. E. ed.) 270-279. Igaku Shoin Ltd. Tokyo. 1975.
12) HANSEL, W., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 223-265. Academic Press. New York. 1959.
13) BODA, J. M., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 335-357. Academic Press. New York. 1959.
14) HAFEZ, E. S. E., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed). 61-80. Lea and Febiger. Philadelphia. 1968.
15) DAY, B. N., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed.) 279-288. Lea and Febiger. Philadelphia. 1968.
16) CHANNING, C. P. and S. KAMMERMAN, Endocrinology, 92: 531-540. 1973.
17) LEE, C. Y., Endocrinology, 99: 42-48. 1976.
18) RYAN, R. J. and C. Y. LEE, Biol. Reprod., 14: 16-29. 1976.
19) PORTER, K., D. PRESCOTT and J. FRYE, J. Cell Biol., 57: 815-836. 1973.
20) BOYDE, A., R. A. WEISS and P. VESELY, Exp. Cell Res., 71: 313-324. 1972.
21) WILLOCH, M., Acta Pathol. Microbiol. Scand., 71: 35-45. 1967.
22) FOLLETT, E. A. C. and R. D. GOLDMAN, Exp. Cell Res., 59: 124-136. 1970.
23) ANDREW S, P. M. and K. R. PORTER, Anat. Rec., 177: 409-426. 1973.
24) EILER, H. and A. V. NALBANDOV, Endocrinology, 100: 331-338. 1977.

Right : 1) SHORT, R. V., Recent Progr. Hormone Res., 20: 303-340. 1965.
2) SAVARD, K., J. M. MARSH and B. F. RICE, Recent Progr. Hormone Res., 21: 285-365. 1965.
3) DORFMAN, R. I., in Handbook of Physiology. section 7. vol. 2. part 1. (GREED, R. O. and E. B. ASTDWOOD, eds.) 537-546. Williams and Wilkins Comp. Baltimore. 1973.
4) BJERSING, L., in The Ovary. 2nd ed. Vol. 1. (ZUCKERMAN, L. and B. J. WEIR, eds.) 303-391. Academic Press. New York. 1977.
5) ECKSTEIN, P., in The Ovary. 2nd ed. vol. 2. (ZUCKERMAN, L. and B. J. WEIR, eds.) 275-313. Academic Press. New York. 1977.
6) CHANNING, C. P., F. W. SCHAERF, L. D. ANDERSON and A. TSAFRIRI, Reproductive Physiology. International Review of Physiology. vol. 22 (GREEP, R. O., ed.) 117-201. University Park Press. Baltimore. 1980.
7) STEINETZ, B. G., in Handbook of Physiology. section 7. vol. 2. part 1. (GREEP, R. O. and E. B. ASTWOOD, eds.) 439-466. Williams and Wilkins Comp. Baltimore. 1973.
8) MCNATTY,K. P., in The Vertebrate Ovary. (JONES, R. E. ed.) 215-259. Plenum Press. New York. 1978.
9) CHANG, S. C. S. and R. J. RYAN, Mayo Clinic Proceedings, 51: 621-623. 1976.
10) CHANG, S. C. S., W. ANDERSON, J. C. LEWIS, R. J. RYAN and Y. H. KANG, Biol. Reprod., 16: 349-357. 1977.
11) GWATKIN, R. B. L. and H. W. CARTER, in Scanning Electron Microscopic Atlas of Mammalian Reproduction (HAFEZ, E. S. E. ed.) 270-279. Igaku Shoin Ltd. Tokyo. 1975.
12) HANSEL, W., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 223-265. Academic Press. New York. 1959.
13) BODA, J. M., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 335-357. Academic Press. New York. 1959.
14) HAFEZ, E. S. E., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed). 61-80. Lea and Febiger. Philadelphia. 1968.
15) DAY, B. N., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed.) 279-288. Lea and Febiger. Philadelphia. 1968.
16) CHANNING, C. P. and S. KAMMERMAN, Endocrinology, 92: 531-540. 1973.
17) LEE, C. Y., Endocrinology, 99: 42-48. 1976.
18) RYAN, R. J. and C. Y. LEE, Biol. Reprod., 14: 16-29. 1976.
19) PORTER, K., D. PRESCOTT and J. FRYE, J. Cell Biol., 57: 815-836. 1973.
20) BOYDE, A., R. A. WEISS and P. VESELY, Exp. Cell Res., 71: 313-324. 1972.
21) WILLOCH, M., Acta Pathol. Microbiol. Scand., 71: 35-45. 1967.
22) FOLLETT, E. A. C. and R. D. GOLDMAN, Exp. Cell Res., 59: 124-136. 1970.
23) ANDREW S, P. M. and K. R. PORTER, Anat. Rec., 177: 409-426. 1973.
24) EILER, H. and A. V. NALBANDOV, Endocrinology, 100: 331-338. 1977.

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

The surface of granulosa cells from porcine ovarian follicles was examined by scanning electron microscopy. The granulosa cells were generally round in shape. The surface of the cells from small and medium-sized follicles was relatively smooth and most of them were lacking microvilli on the surface. On the contrary, the cells from large follicles (6-12mm), particularly those close to the lumen of the follicle, were generally covered with numerous microvilli. These results indicate that the appearance of microvilli on the surface of granulosa cells may be related to the developmental process of the follicle. No morphological differences were observed between prepuberal and mature animals concerning the surface of the cells from follicles within the same sizeclass. No significant differences were found among the stages of the estrous cycle concerning the surface features of cells from follicles within the same size-class. The surface of collapsed granulosa cells in early cystic follicles was scattered with a few microvilli. The inner lining of the advanced cystic follicle became covered with fibrin or collagen fibers after granulosa cells were completely lost.

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