Relation Between Free Amino Acid Type and Activity of Transaminase, Concentration of Glutathione in Red Blood Cells in Sheep

Abstract

For clarify the biochemical traits on the free amino acid type of red blood cells in sheep, in the present studies, the activity of transaminase and the concentration of glutathion reduced form (GSH) were compared between both amino acid types, Lys+ and Lys^-type, which were classified by the paper electrophoresis. 1) The zymograms of three kinds of transaminase, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and aromatic amino acid transaminase, in sheep red blood cells were observed by starch gel electrophoresis. The zymograms of GOT and aromatic amino acid transaminase appeared to be single band, and GPT zymograms was recognized with two zones. However, there were no individual differences in the zymograms, respectively. 2) GOT and GPT activities in red blood cells were compared with Lys⁺ and Lys^- type in Finnish Landrace sheep. GOT activity in Lys^-type of red blood cells was higher in 4.4 fold(177.82±16.11 Unit of Reitman-Frankel) than that of Lys⁺type (40.6±0.46 Unit of Reitman-Frankel).GSH concentration in red blood cells of Lys^-type was higher in 5.3 fold(54.12±3.47mg/100ml)than that of Lys⁺ type(10.23±0.18mg/100ml). The concentration of GSH in red blood cells of Lys^-type was compared among Finnish Landrace, Suffolk and Hybrid from matings between both breeds. In the result, these concentrations were 54.12±3.47, 72.29±2.15 and 62.52±2.54 (mg/100ml), respectively, and it is assumed clearly that the concentration of GSH was difference owing to breeds.