DNA Fingerprint using Bacteriophage M 13 Probe in Chickens

Abstract

Genomic DNA was extracted from three breeds of chickens and digested with restriction enzyme, Hae III. DNA fingerprinting was applied to the digested DNA using 15 base pair repeats in the bacteriophage M13 as a probe. These repeats detected 25 to 35 bands, between 0.5kbp and 10kbp, in each chicken examined. Although banding pattern in the DNA fingerprint is specific to each individual, several common bands were found within the same breed. A parentage test was carried out with parent-offspring DNA fingerprints. All bands of F1 embryo were always originated from either parent. The similar banding pattern in DNA fingerprint was observed between the germ cell and the differentiated cell in the same individual. From the above results, the method described here seemed to be useful for identifying the individual and parent-offspring.