細菌感染状況が試料採取時刻による乳汁の乳房炎諸指標の変動に及ぼす影響

青木 康浩; 野附 巖; 中野 光志; 市川 忠雄

doi:10.2508/chikusan.61.1084

Influence of Infectious Status of Quarters on Variation Patterns of Mastitis Indicators Relating to Sampling Time

Yasuhiro AOKI, Iwao NOTSUKI, Terushi NAKANO, Tadao ICHIKAWA

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Keywords: somatic cells in milk, electrical conductivity, NAGase activity, udder health

JOURNAL FREE ACCESS

1990 Volume 61 Issue 12 Pages 1084-1094

DOI https://doi.org/10.2508/chikusan.61.1084

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Published: December 25, 1990 Received: June 11, 1990 Available on J-STAGE: March 10, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 10, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : In order to establish the method for detecting subclinical mastitis, the experiments were carried out to examine the variation in the several mastitis indicators and the effect of infectious status on that variation patterns. From 16 (Experiment 1) and 38 (Experi-ment 2) quarters, of which infectious status had been determined, milk samples were collected at intervals of 2 hours during 12 (Exp. 1) or 24 (Exp. 2) hours and examined somatic cell count (SCC), distribution percentages of the components of somatic cells (epithelial cell, lymphocyte, neutrophil, monocyte and others), electrical conductivity (EC; Exp. 1 only) and N-acetyl-β-D-glucosaminidase (NAGase) activity (Exp. 2 only).
Results were summarized as follows. (1) SCC in milk samples from each quarter abruptly increased to the maximum at the first 2 hours after milking and, thereafter, declined gradually toward the next milking, regardless of the infectious status of the quarters. The ratio to SCC in foremilk sample at the routine milking were 4.2, 3.5, 2.5 and 1.8 times at 2, 4, 6 and 8 hours after milking, respectively. (2) In foremilk samples from uninfected quarters, epithelial cells were dominant. At 2 hours after milking, however, the distribution percentages of neutrophil, lymphocyte and monocyte increased and that of epithelial cell decreased, and these percentages returned to the basal state toward next milking. On the other hand, regarding to the milk from infected quarters by major pathogens, the percentages of neutrophil and monocytes were high at every sampling time. (3) The variation pattern of EC relating to sampling time and infectious status was not shown clearly. It was guessed that the EC values were modified by milk fat. (4) The greatest difference of NAGase activity between groups of quarters according to infectious status was observed at 4 hours after milking, and it was shown that the NAGase activity was significantly higher as infectious status of quarters became more severe. These facts suggest that, determining the state of health of each quarter from data of milk samples obtained between routine milking times, it must be regarded that, even if the quarters are not infected by any pathogens, SCC will increase as mentioned above and the distribution percentages of cells from blood become higher than routine milking time. Also, it is suggested that, for determining the state of udder health more exactly, it is useful to examine NAGase activity, as well as SCC.
Right : In order to establish the method for detecting subclinical mastitis, the experiments were carried out to examine the variation in the several mastitis indicators and the effect of infectious status on that variation patterns. From 16 (Experiment 1) and 38 (Experi-ment 2) quarters, of which infectious status had been determined, milk samples were collected at intervals of 2 hours during 12 (Exp. 1) or 24 (Exp. 2) hours and examined somatic cell count (SCC), distribution percentages of the components of somatic cells (epithelial cell, lymphocyte, neutrophil, monocyte and others), electrical conductivity (EC; Exp. 1 only) and N-acetyl-β-D-glucosaminidase (NAGase) activity (Exp. 2 only).
Results were summarized as follows. (1) SCC in milk samples from each quarter abruptly increased to the maximum at the first 2 hours after milking and, thereafter, declined gradually toward the next milking, regardless of the infectious status of the quarters. The ratio to SCC in foremilk sample at the routine milking were 4.2, 3.5, 2.5 and 1.8 times at 2, 4, 6 and 8 hours after milking, respectively. (2) In foremilk samples from uninfected quarters, epithelial cells were dominant. At 2 hours after milking, however, the distribution percentages of neutrophil, lymphocyte and monocyte increased and that of epithelial cell decreased, and these percentages returned to the basal state toward next milking. On the other hand, regarding to the milk from infected quarters by major pathogens, the percentages of neutrophil and monocytes were high at every sampling time. (3) The variation pattern of EC relating to sampling time and infectious status was not shown clearly. It was guessed that the EC values were modified by milk fat. (4) The greatest difference of NAGase activity between groups of quarters according to infectious status was observed at 4 hours after milking, and it was shown that the NAGase activity was significantly higher as infectious status of quarters became more severe.
These facts suggest that, determining the state of health of each quarter from data of milk samples obtained between routine milking times, it must be regarded that, even if the quarters are not infected by any pathogens, SCC will increase as mentioned above and the distribution percentages of cells from blood become higher than routine milking time. Also, it is suggested that, for determining the state of udder health more exactly, it is useful to examine NAGase activity, as well as SCC.

Date of correction: March 10, 2008 Reason for correction: - Correction: AFFILIATION Details: Wrong :
1) Faculty of Agriculture, Tokyo University of Agriculture and Technology
2) Koibuchi Agriculture College, Uchihara-cho
3) School of Veterinary and Animal Science, Kitasato University

Right :
1) Faculty of Agriculture, Tokyo University of Agriculture and Technology
2) Koibuchi Agriculture College
3) School of Veterinary and Animal Science, Kitasato University

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

In order to establish the method for detecting subclinical mastitis, the experiments were carried out to examine the variation in the several mastitis indicators and the effect of infectious status on that variation patterns. From 16 (Experiment 1) and 38 (Experi-ment 2) quarters, of which infectious status had been determined, milk samples were collected at intervals of 2 hours during 12 (Exp. 1) or 24 (Exp. 2) hours and examined somatic cell count (SCC), distribution percentages of the components of somatic cells (epithelial cell, lymphocyte, neutrophil, monocyte and others), electrical conductivity (EC; Exp. 1 only) and N-acetyl-β-D-glucosaminidase (NAGase) activity (Exp. 2 only).
Results were summarized as follows. (1) SCC in milk samples from each quarter abruptly increased to the maximum at the first 2 hours after milking and, thereafter, declined gradually toward the next milking, regardless of the infectious status of the quarters. The ratio to SCC in foremilk sample at the routine milking were 4.2, 3.5, 2.5 and 1.8 times at 2, 4, 6 and 8 hours after milking, respectively. (2) In foremilk samples from uninfected quarters, epithelial cells were dominant. At 2 hours after milking, however, the distribution percentages of neutrophil, lymphocyte and monocyte increased and that of epithelial cell decreased, and these percentages returned to the basal state toward next milking. On the other hand, regarding to the milk from infected quarters by major pathogens, the percentages of neutrophil and monocytes were high at every sampling time. (3) The variation pattern of EC relating to sampling time and infectious status was not shown clearly. It was guessed that the EC values were modified by milk fat. (4) The greatest difference of NAGase activity between groups of quarters according to infectious status was observed at 4 hours after milking, and it was shown that the NAGase activity was significantly higher as infectious status of quarters became more severe.
These facts suggest that, determining the state of health of each quarter from data of milk samples obtained between routine milking times, it must be regarded that, even if the quarters are not infected by any pathogens, SCC will increase as mentioned above and the distribution percentages of cells from blood become higher than routine milking time. Also, it is suggested that, for determining the state of udder health more exactly, it is useful to examine NAGase activity, as well as SCC.

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