Human Erythropoietin Gene Expression in Primary Cultured Oviduct Cells from Estrogen-Stimulated Chicks

Abstract

The present study was conducted to investigate whether or not the human erythropoietin gene is expressed in primary cultured oviduct cells from estrogen-stimulated chicks. The erythropoietin gene was transfected by calcium phosphate precipitation, and erythropoietin mRNA level was determined by RNA:RNA solution hybridization during the culture periods for up to 72hrs. The results indicated that the human erythropoietin gene was actually transcribed in the chicken oviduct cells, and erythropoietin mRNA concentration in-creased on the basis of both per cells and per total nucleic acids as the culture period went by, reaching maximal values at 72hrs.