核移植由来黒毛和種牛におけるマイクロサテライトDNA多型領域の解析

矢澤 慈人; 青柳 敬人; 溝下 和則; 猪八重 悟; 杉本 喜憲

doi:10.2508/chikusan.68.1166

Analysis of Microsatellite DNA Polymorphism of Japanese Black Cattle Produced by Nuclear Transfer

Shigeto YAZAWA, Yoshito AOYAGI, Kazunori MIZOSHITA, Satoru INOHAE, Yoshinori SUGIMOTO

Author information

Keywords: Microsatellite, Polymorphism, Nuclear transfer, Cattle

JOURNAL FREE ACCESS

1997 Volume 68 Issue 12 Pages 1166-1169

DOI https://doi.org/10.2508/chikusan.68.1166

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Published: December 25, 1997 Received: July 31, 1997 Available on J-STAGE: March 10, 2008 Accepted: - Advance online publication: - Revised: -
Correction information
Date of correction: March 10, 2008 Reason for correction: - Correction: CITATION Details: Wrong : 2) Aoyagi Y, Konishi M, Wada T, Takedomi T. Unaged bovine oocytes successfully develop to blastocysts after parthenogenic activation or nuclear transfer. Theriogenology, 41: 157 (abstr.). 1994.
4) Inoue-Murayama M, Hirano T, Watanabe T, Mizoshita K, Yamakuchi H, Nakane S, Sugimoto Y. Individual identification and paternity control of Japanese Black cattle based on microsatellite polymorphism. Anim. Sci.Technol. (Jpn.), 68: 443-449. 1997.
6) Prather RS, Barnes FL, Sims MM, Robl JM, Eyestone WH, First NL. Nuclear transplantation in the bovine embryo: assessment of donor nuclei and recipient oocyte. Biol. Reprod., 37: 859-866. 1987.
8) Willadsen JM, Williams JD, Bondioli KR, Looney CR, Westhusin ME, McCalla DF. Comparison of birth weight and growth characteristics of bovine calves produced by nuclear transfer (cloning), embryo transfer and natural mating. Anim. Reprod. Sci., 38: 73-84. 1995.
9) Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS. Viable offspring derived from fetal and adult mammalian cells. Nature, 385: 810-813. 1997.

Right : 2) Aoyagi Y, Konishi M, Wada T, Takedomi T. Unaged bovine oocytes successfully develop to blastocysts after parthenogenic activation or nuclear transfer. Theriogenology, 41: 157 (abstr.). 1994.
4) Inoue-Murayama M, Hirano T, Watanabe T, Mizoshita K, Yamakuchi H, Nakane S, Sugimoto Y. Individual identification and paternity control of Japanese Black cattle based on microsatellite polymorphism. Anim. Sci.Technol. (Jpn.), 68: 443-449. 1997.
6) Prather RS, Barnes FL, Sims MM, Robl JM, Eyestone WH, First NL. Nuclear transplantation in the bovine embryo: assessment of donor nuclei and recipient oocyte. Biol. Reprod., 37: 859-866. 1987.
8) Willadsen JM, Williams JD, Bondioli KR, Looney CR, Westhusin ME, McCalla DF. Comparison of birth weight and growth characteristics of bovine calves produced by nuclear transfer (cloning), embryo transfer and natural mating. Anim. Reprod. Sci., 38: 73-84. 1995.
9) Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS. Viable offspring derived from fetal and adult mammalian cells. Nature, 385: 810-813. 1997.

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 10, 2008 Reason for correction: - Correction: CITATION Details: Wrong : 2) Aoyagi Y, Konishi M, Wada T, Takedomi T. Unaged bovine oocytes successfully develop to blastocysts after parthenogenic activation or nuclear transfer. Theriogenology, 41: 157 (abstr.). 1994.
4) Inoue-Murayama M, Hirano T, Watanabe T, Mizoshita K, Yamakuchi H, Nakane S, Sugimoto Y. Individual identification and paternity control of Japanese Black cattle based on microsatellite polymorphism. Anim. Sci.Technol. (Jpn.), 68: 443-449. 1997.
6) Prather RS, Barnes FL, Sims MM, Robl JM, Eyestone WH, First NL. Nuclear transplantation in the bovine embryo: assessment of donor nuclei and recipient oocyte. Biol. Reprod., 37: 859-866. 1987.
8) Willadsen JM, Williams JD, Bondioli KR, Looney CR, Westhusin ME, McCalla DF. Comparison of birth weight and growth characteristics of bovine calves produced by nuclear transfer (cloning), embryo transfer and natural mating. Anim. Reprod. Sci., 38: 73-84. 1995.
9) Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS. Viable offspring derived from fetal and adult mammalian cells. Nature, 385: 810-813. 1997.

Right : 2) Aoyagi Y, Konishi M, Wada T, Takedomi T. Unaged bovine oocytes successfully develop to blastocysts after parthenogenic activation or nuclear transfer. Theriogenology, 41: 157 (abstr.). 1994.
4) Inoue-Murayama M, Hirano T, Watanabe T, Mizoshita K, Yamakuchi H, Nakane S, Sugimoto Y. Individual identification and paternity control of Japanese Black cattle based on microsatellite polymorphism. Anim. Sci.Technol. (Jpn.), 68: 443-449. 1997.
6) Prather RS, Barnes FL, Sims MM, Robl JM, Eyestone WH, First NL. Nuclear transplantation in the bovine embryo: assessment of donor nuclei and recipient oocyte. Biol. Reprod., 37: 859-866. 1987.
8) Willadsen JM, Williams JD, Bondioli KR, Looney CR, Westhusin ME, McCalla DF. Comparison of birth weight and growth characteristics of bovine calves produced by nuclear transfer (cloning), embryo transfer and natural mating. Anim. Reprod. Sci., 38: 73-84. 1995.
9) Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS. Viable offspring derived from fetal and adult mammalian cells. Nature, 385: 810-813. 1997.

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 10, 2008 Reason for correction: - Correction: AUTHOR Details: Wrong : Shigeto YAZAWA¹⁾, Yoshito AOYAGI¹⁾, Kazunori MIZOSHITA²⁾, Satoru INOHAE²⁾, Yoshinori SUGIMOTO³⁾
Right : Shigeto YAZAWA¹⁾, Yoshito AOYAGI¹⁾, Kazunori MIZOSHITA²⁾, Satoru INOHAE²⁾⁴⁾, Yoshinori SUGIMOTO³⁾

Date of correction: March 10, 2008 Reason for correction: - Correction: AFFILIATION Details: Wrong :
1) Central Research Institute for Feed and Livestock Zen-Noh
2) CatUe Breeding Development Institute of Kagoshima Prefcture
3) Shirakawa Institute of Animal Genetics, Livestock Technology Association of Japan

Right :
1) Central Research Institute for Feed and Livestock Zen-Noh
2) CatUe Breeding Development Institute of Kagoshima Prefeture
3) Shirakawa Institute of Animal Genetics, Livestock Technology Association of Japan
4) Aira Livestock Hygien Center

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

Genetic identity among the cattle produced by nuclear transfer was investigated by the genotyping system with 23 microsatellite markers, which is able to distinguish 31 trillion individuals and discriminate 37 million sires. Two groups (clone sets) of cattle, each consisting of 5 individuals cloned from one embryo, were analyzed with leukocyte DNA collected at the age of 10 (male; No. 45-49) and 15 months (female; No. 186-190), respectively. The genotypes judged by the 23 microsatellite markers were identical in each clone set. Thus, it is suggested that the genomic DNA of the cattle cloned from the same embryo are genetically identical.

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Correction information

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