A New Screening Method for Probiotic Lactic Acid Bacteria in the Lactobacillus acidophilus Group by Using Carbohydrate Probes

Tadao SAITO; Atsushi MATSUMURA; Haruki KITAZAWA; Yasushi KAWAI; Takatoshi ITOH; Honoh HASHIBA

doi:10.2508/chikusan.71.103

Tadao SAITO, Atsushi MATSUMURA, Haruki KITAZAWA, Yasushi KAWAI, Takatoshi ITOH, Honoh HASHIBA

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Keywords: Lactobacillus acidophilus, Cell-surface lectin, Biotinylated carbohydrate probe, Sugar chain, Surface-layer protein

JOURNAL FREE ACCESS

2000 Volume 71 Issue 1 Pages 103-108

DOI https://doi.org/10.2508/chikusan.71.103

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Published: January 25, 2000 Received: August 19, 1999 Available on J-STAGE: March 10, 2008 Accepted: November 10, 1999 Advance online publication: - Revised: -
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Date of correction: March 10, 2008 Reason for correction: - Correction: CITATION Details: Wrong : 1) Abramenko IV, Gluzman DF, Korchagina EYu, Zemlyanukhina TV, Bovin NV. Oligosaccharidebinding molecules on the surface of human hemopoietic and lymphoid cells. FEBS Letters, 307: 283-286. 1992.
2) Benno Y, Endo K, Mizutani T, Namba Y, Komori T, Mitsuoka T. Comparison of fecal microflora of elderly persons in rural and urban areas of Japan. Applied and Environmental Microbiology, 55: 1100-1105. 1989.
3) Coppee I, Gabius HJ, Danguy A. Histochemical analysis of carbohydrate moieties and sugar-specific acceptors in the kidneys of the laboratory mouse and the golden spiny mouse (Acomys russatus). Histology and Histopathology, 8: 673-683. 1993.
4) Johnson JL, Phelps CF, Cummins CS, London J, Gasser F. Taxonomy of the Lactobacillus acidophilus group. International Journal of Systematic Bacteriology, 30: 53-68. 1980.
5) Lauer E, Helming C, Kandler O. Heterogeneity of the species Lactobacillus acidophilus (Moro) Hansen and Moquot as revealed by biochemical characteristics and DNA-DNA hybridisation. Zentralblatt fur Bakteriologie, Mikrobiologie, and Hygiene. 1. Abt, Originale, C, Allgemeine, angewandte, und okologische Mikrobiologie C1: 150-168. 1980.
6) Mukai T, Arihara K, Itoh H. Lectin-like activity of Lactobacillus acidophilus strain JCM1026. FEMS Microbioly Letters, 98: 71-74. 1992.
7) Podolsky DK. Oligosaccharide structure of human colonic mucin. Journal of Biological Chemistry, 260: 8262-8271. 1985.
8) Sellars RL. Acidophilus products. In: Therapeutic Properties of Fermented Milks. (Robinson RK. ed) 81-116. Elsevier. New York. 1991.
9) Takahashi N, Saito T, Ohwada S, Ota H, Hashiba H, Itoh T. A new screening method for the selection of Lactobacillus acidophilus group lactic acid bacteria with high adhesion to human colonic mucosa. Bioscience, Biotechnology and Biochemistry, 60: 1434-1438. 1996.
10) Yamada M, Saito T, Toba T, Kitazawa H, Uemura J, Itch T. Hemagglutination activity of Lactobacillus acidophilus group lactic acid bacteria. Bioscience, Biotechnology and Biochemistry, 58: 910-915. 1994.

Right : 1) Abramenko IV, Gluzman DF, Korchagina EYu, Zemlyanukhina TV, Bovin NV. Oligosaccharidebinding molecules on the surface of human hemopoietic and lymphoid cells. FEBS Letters, 307: 283-286. 1992.
2) Benno Y, Endo K, Mizutani T, Namba Y, Komori T, Mitsuoka T. Comparison of fecal microflora of elderly persons in rural and urban areas of Japan. Applied and Environmental Microbiology, 55: 1100-1105. 1989.
3) Coppee I, Gabius HJ, Danguy A. Histochemical analysis of carbohydrate moieties and sugar-specific acceptors in the kidneys of the laboratory mouse and the golden spiny mouse (Acomys russatus). Histology and Histopathology, 8: 673-683. 1993.
4) Johnson JL, Phelps CF, Cummins CS, London J, Gasser F. Taxonomy of the Lactobacillus acidophilus group. International Journal of Systematic Bacteriology, 30: 53-68. 1980.
5) Lauer E, Helming C, Kandler O. Heterogeneity of the species Lactobacillus acidophilus (Moro) Hansen and Moquot as revealed by biochemical characteristics and DNA-DNA hybridisation. Zentralblatt fur Bakteriologie, Mikrobiologie, and Hygiene. 1. Abt, Originale, C, Allgemeine, angewandte, und okologische Mikrobiologie C1: 150-168. 1980.
6) Mukai T, Arihara K, Itoh H. Lectin-like activity of Lactobacillus acidophilus strain JCM1026. FEMS Microbioly Letters, 98: 71-74. 1992.
7) Podolsky DK. Oligosaccharide structure of human colonic mucin. Journal of Biological Chemistry, 260: 8262-8271. 1985.
8) Sellars RL. Acidophilus products. In: Therapeutic Properties of Fermented Milks. (Robinson RK. ed) 81-116. Elsevier. New York. 1991.
9) Takahashi N, Saito T, Ohwada S, Ota H, Hashiba H, Itoh T. A new screening method for the selection of Lactobacillus acidophilus group lactic acid bacteria with high adhesion to human colonic mucosa. Bioscience, Biotechnology and Biochemistry, 60: 1434-1438. 1996.
10) Yamada M, Saito T, Toba T, Kitazawa H, Uemura J, Itch T. Hemagglutination activity of Lactobacillus acidophilus group lactic acid bacteria. Bioscience, Biotechnology and Biochemistry, 58: 910-915. 1994.

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 10, 2008 Reason for correction: - Correction: CITATION Details: Wrong : 1) Abramenko IV, Gluzman DF, Korchagina EYu, Zemlyanukhina TV, Bovin NV. Oligosaccharidebinding molecules on the surface of human hemopoietic and lymphoid cells. FEBS Letters, 307: 283-286. 1992.
2) Benno Y, Endo K, Mizutani T, Namba Y, Komori T, Mitsuoka T. Comparison of fecal microflora of elderly persons in rural and urban areas of Japan. Applied and Environmental Microbiology, 55: 1100-1105. 1989.
3) Coppee I, Gabius HJ, Danguy A. Histochemical analysis of carbohydrate moieties and sugar-specific acceptors in the kidneys of the laboratory mouse and the golden spiny mouse (Acomys russatus). Histology and Histopathology, 8: 673-683. 1993.
4) Johnson JL, Phelps CF, Cummins CS, London J, Gasser F. Taxonomy of the Lactobacillus acidophilus group. International Journal of Systematic Bacteriology, 30: 53-68. 1980.
5) Lauer E, Helming C, Kandler O. Heterogeneity of the species Lactobacillus acidophilus (Moro) Hansen and Moquot as revealed by biochemical characteristics and DNA-DNA hybridisation. Zentralblatt fur Bakteriologie, Mikrobiologie, and Hygiene. 1. Abt, Originale, C, Allgemeine, angewandte, und okologische Mikrobiologie C1: 150-168. 1980.
6) Mukai T, Arihara K, Itoh H. Lectin-like activity of Lactobacillus acidophilus strain JCM1026. FEMS Microbioly Letters, 98: 71-74. 1992.
7) Podolsky DK. Oligosaccharide structure of human colonic mucin. Journal of Biological Chemistry, 260: 8262-8271. 1985.
8) Sellars RL. Acidophilus products. In: Therapeutic Properties of Fermented Milks. (Robinson RK. ed) 81-116. Elsevier. New York. 1991.
9) Takahashi N, Saito T, Ohwada S, Ota H, Hashiba H, Itoh T. A new screening method for the selection of Lactobacillus acidophilus group lactic acid bacteria with high adhesion to human colonic mucosa. Bioscience, Biotechnology and Biochemistry, 60: 1434-1438. 1996.
10) Yamada M, Saito T, Toba T, Kitazawa H, Uemura J, Itch T. Hemagglutination activity of Lactobacillus acidophilus group lactic acid bacteria. Bioscience, Biotechnology and Biochemistry, 58: 910-915. 1994.

Right : 1) Abramenko IV, Gluzman DF, Korchagina EYu, Zemlyanukhina TV, Bovin NV. Oligosaccharidebinding molecules on the surface of human hemopoietic and lymphoid cells. FEBS Letters, 307: 283-286. 1992.
2) Benno Y, Endo K, Mizutani T, Namba Y, Komori T, Mitsuoka T. Comparison of fecal microflora of elderly persons in rural and urban areas of Japan. Applied and Environmental Microbiology, 55: 1100-1105. 1989.
3) Coppee I, Gabius HJ, Danguy A. Histochemical analysis of carbohydrate moieties and sugar-specific acceptors in the kidneys of the laboratory mouse and the golden spiny mouse (Acomys russatus). Histology and Histopathology, 8: 673-683. 1993.
4) Johnson JL, Phelps CF, Cummins CS, London J, Gasser F. Taxonomy of the Lactobacillus acidophilus group. International Journal of Systematic Bacteriology, 30: 53-68. 1980.
5) Lauer E, Helming C, Kandler O. Heterogeneity of the species Lactobacillus acidophilus (Moro) Hansen and Moquot as revealed by biochemical characteristics and DNA-DNA hybridisation. Zentralblatt fur Bakteriologie, Mikrobiologie, and Hygiene. 1. Abt, Originale, C, Allgemeine, angewandte, und okologische Mikrobiologie C1: 150-168. 1980.
6) Mukai T, Arihara K, Itoh H. Lectin-like activity of Lactobacillus acidophilus strain JCM1026. FEMS Microbioly Letters, 98: 71-74. 1992.
7) Podolsky DK. Oligosaccharide structure of human colonic mucin. Journal of Biological Chemistry, 260: 8262-8271. 1985.
8) Sellars RL. Acidophilus products. In: Therapeutic Properties of Fermented Milks. (Robinson RK. ed) 81-116. Elsevier. New York. 1991.
9) Takahashi N, Saito T, Ohwada S, Ota H, Hashiba H, Itoh T. A new screening method for the selection of Lactobacillus acidophilus group lactic acid bacteria with high adhesion to human colonic mucosa. Bioscience, Biotechnology and Biochemistry, 60: 1434-1438. 1996.
10) Yamada M, Saito T, Toba T, Kitazawa H, Uemura J, Itch T. Hemagglutination activity of Lactobacillus acidophilus group lactic acid bacteria. Bioscience, Biotechnology and Biochemistry, 58: 910-915. 1994.

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 10, 2008 Reason for correction: - Correction: DTPUB Details: Right : 20000125

Date of correction: March 10, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

To select probiotic strains of the Lactobacillus (L.) acidophilus group with high adhesion to human intestinal tracts for developing the functional yogurt, a new screening method using biotinylated carbohydrate probe (BCP) was introduced. After incubation of living cells of 30 strains of the L. acidophilus group with 5 kinds of BCPs which possess representative partial structures of the sugar chains constituting of human colonic mucin, the interaction between cell-surface lectins and BCP were evaluated. The highest reactivity was detected in 19 strains (about 63%) with a trisaccharide probe (GalNAcα (1→3)[Fucα (1→2)] Galβ 1→). The cell-surface lectin activity was first clearly confirmed in the B group of the L. acidophilus group. The adhesion evaluation score (AE score, total sum of each absorbance of 5 BCPs samples) is considered to be useful as an indicator for selection of the probiotic strains which have high adhesiveness in human gastrointestinal tract.

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