Cloning of the Promoter Region and Expression Analysis of Mouse Insulin Receptor-related Receptor (Irr) Gene

Abstract

In the present study, we isolated promoter region of mouse insulin receptor-related receptor (Irr) gene and analyzed its expression in various mouse tissues and embryos in different stages. The nucleotide sequence of the promoter region of the Irr gene showed the presence of putative promoter elements and binding sites for trans-acting factors. The expression analysis revealed the strong expression in kidney, stomach, and embryo at the stage of 14.5 days. These findings were basically consistent with the previously reported findings of rat and human, but we found several differences in the sequence and expression pattern of the gene between mouse and rat or human.