抄録
The 2nd Experiment : The adrenocortical function in experimental renal hypertensive dogs.The author made experimental hypertension in dogs by Goldblatt's or Page's method, and measured 17-KS excretion in urine comparing with histochemical examination of the adrenal cortex.EXPERIMENTAL PROCEDURE 1. Goldblatt's method : The main renal arteries on both sides were ligated in 7 of 12 dogs. In other 5 dogs which were nephrectomized unilaterally, the main renal artery of the remaining kidney was ligated. After the operation they were observed for 38 to 284 days.2. Page's method : One side of kidney was covered with cellophane or silk cloth in 8 dogs, and then other side was treated with the same procedure. After the operation they were observed for 28 to 107 day.RESULTS 1) Blood pressure a) Blood pressure of normal dogs. According to the examination of 46 normal dogs, blood pressure showed 90-150 mmHg, and on the average 125±9.7 mmHg, 40% of 46 dogs showed 120-130 mmHg, 20% 110-120 mmHg, and 20% 130-140 mmHg.b) Blood pressure of experimental dogs. The elevation of blood pressure was classified into three classes, i.e. slight elevation (below 150 mmHg), moderate (150-170 mmHg) and severe (above 170 mmHg).(α) In 12 Goldblatt's dogs two showed slight and four severe hypertension. Ratio of success in production of hypertension was 50%.(β) In 8 Page's dogs one showed slight, one moderate and two severe hypertension. Ratio of success was therefore 50%.2) 17-KS excretion in urine a) 17-KS excretion in urine of normal dogs. In 16 males it showed 0.1-0.97 mg/day and in 12 females 0.04-0.75 mg/day.b) 17-KS excretion in urine of experimental dogs.(α) Goldblatt's dogs. On the day of operation, it showed a marked decrease and then gradually increased to reach the highest value. After that it decreased and restored the preoperative value. 17-KS had nothing to do with blood pressure.(β) Page's dog. On the day of operation, it showed a marked low value and then gradually increased to reach the highest level. After that it decreased to the preoperative level. 17-KS level and blood pressure did not necessarily go parallel.3) Histochemical study of the adrenal cortex Parallel to the elevation of blood pressure the zona glomerulosa showed a hypertrophy, i.e. hyperfunction, and the zona fasciculata showed the findings of stress. From the findings of zona glomerulosa this layer or the adrenal cortex seems to be related to the renal hypertension.The 3rd Experiment : The functions of adrenal cortex in experimental DCA hypertensive rats.The author treated rats with DCA and salt to make hypertension, and the measurement of 17-KS excretion in urine and histochemical examination of adrenal cortex were made.EXPERIMENTAL PROCEDURE The author used 9 male rats, weighing 130-240 g, which were divided into three groups, each group consisting of three rats. The first and second groups were injected subcutaneously with 5 mg of DCA every second day. And the first and third groups were given 1% salt solution to drink and the second group distilled water.RESULTS 1) Blood pressure a) Blood pressure of normal rats. Mean value of 74 normal rats was 106.8 mmHg and standard deviation 48 mmHg.b) Blood pressure of experimental rats. In the first and second groups, blood pressure began to rise on the 10th day. On the 40th day it reached 200 mmHg in the first group, but 147 mmHg in the second group, which therefore showed a much slighter increase than in the first group. In the third group, no change was observed in blood pressure. Before death, the blood pressure was measured at 110 mmHg.2) 17-KS excretion in urine a) 17-KS excretion in urine of normal rats. 3 days' urine of a pair of rats was used to measure its 17-KS excretion in 26 normal rats and 2 days' urine of each three rats in other 9. This measurement is, therefore, considered as 17-KS excretion of 6 rats in one day's urine, which showed 14 to 95γ.
