実験的心筋硬塞において各種薬剤が心筋Aspartate : Aminotransferase(2.6.1.1)-Isozymeの変動におよぼす影響に関する研究

抄録

Previously KATUNUMA has shown that GOT from pig liver consists of two types of isozyme, the one being localized in cell supernatant (GOTs) and the other in mitocondria(GOTM), and subsequently purified them to almost single protein. Using the technique of colum chromatographic separation, the author elucidated the changes of GOT-isozyme and effects of some medicines on the enzymatic activity in infarcted heart muscles togather with subsequent changes of pattern of ECG in experimental infarction in order to develop the way to their clinical application. Method of the Experiment Experimental myocardial infarction was made by ligating the coronary artery of dogs at the circumflex branch of the left coronary artery. The direct ECG were recorded before or twenty-four hours after occlusion respectively. The dogs were sacrificed for the enzymatic assay. Furthermore, in order to determine the effect of some medicines, medicines were injected intravenously before or immediately after the ligation of coronary artery. Dogs were killed and the removed heart muscle was divided into the infarcted portion and non-infarcted portion. The isozymic activity were measured on the materials obtained from each portion. HUZlNO-KATUNUMA'S method was employed for the separation and the measurement of the isozymic activity. Results 1. GOT-isozyme in Normal Heart Muscles: The ratio of the activity of GOTS to GOTM in normal heart muscles was about 4 to I on the average. 2. Twenty-four Hours after Occlusion: 1) Control groups without medicines The average ratio of the activity of GOTS to GOTM in the non-infarcted portion were almost the same value as that in the normal heart muscles. The average activity of GOTS in the infarcted portion decreased about 70 per cent compared with that in the non-infarcted portion. 2) The effect of some medicines on the GOT-isozymes in the infarcted portion a) Control group (physiological saline solution) The rates of decrease of GOTS were 62.8 per cent and 73.6 per cent respectively, and, in comparison with the control group to which no medicines were administered, any difference could not be observed.