EFFECT OF PHOSPHOLIPASE C ON THE PERMEABILITY OF THE TOAD BLADDER

Abstract

1) Using toad bladder, the effects of phospholipase C on the vasopressin-, cyclic AMP-, and theophylline-activated SCC and osmotic flow were examined. The effect of phospholipase C on the vasopressin-activated urea permeability across the toad bladder was also examined. 2) Phospholipase C inhibited the vasopressin-cyclic AMP-, and theophylline-activated osmotic flow of the toad bladder. 3) Phospholipase C did not affect the vasopressin-, cyclic AMP-. and theophylline activated SCC. 4) Phospholipase C caused no effect on vasopressin-induced increment of the urea permeability across the toad bladder membrane. 5) The results suggest that phospholipase C might affect directly the barrier for water, and shows no effect on the barrier for solutes. Some possible characteristics of the barriers are discussed in the point of view of the "double barrier series theory".