Journal of Japanese Cleft Palate Association
Online ISSN : 2186-5701
Print ISSN : 0386-5185
ISSN-L : 0386-5185
Experimental Study on Cleft Lip and Palate
Preventive Effects of Estradiol on Cleft Lip and/or Palate in A/J mice
Shigeki MIURANagato NATSUMERyusaku HORIUCHIToinoyasu NAKAMURAYoshinori KANOHHiroshi FUJIWARAHiroo FURUKAWAShouji INAGAKITetsuya MARUKAWATsuyoshi KAWAI
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1990 Volume 15 Issue 2 Pages 122-131

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Abstract
We previously found a positive correlation between the prolonged estrous cycle due to agihg and the incidence of cleft lip/palate in A/J mice. It was hypothesized that this correlation may be ascribed to the metabolism of estradiol. We thus carried out the following experiment:
A/J strain mice were used. Females aged 70 days were mated overnight with males, and the day then a vaginal plug was observed was taken as gestation day 0. Pregnant females were injected subcutaneously with 5,12.5,25μg/kg of estradiol benzoate on day 8,9, or 10 of gestation.
All pregnant females were sacrificed on gestation day 18. The numbers of implantation site, resorption, and dead fetus were recorded. Fetuses were removed and examined for gross anomalies. The incidence of cleft lip with cleft palate and that without cleft palate were analysed by Mann-Whitney's U test.
As a result, the incidence of cleft lip with or without cleft palate was significantly reduced on days 8 and 9 of gestation, but their fetal mortality rates showed no significantly change. So, our results suggested that estradiol has a preventive effect on cleft lip and/or palate in A/J mice.
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