Abstract
Culture samples collected at regular intervals during the tank culture of Eremothecium ashbyii were separated into the mycelium and broth. The mycelium was extracted with various solvents and the extracts were examined by paper chromatography and paper electrophoresis. The broth, as such or after concentration, was also investigated by the same methods. Development with the solvent system of butanol-ethanol-water afforded detection of the greatest number of substances in the mycelium and gave the best separation. From the spots fixed by various methods it was found that adenosine was formed besides riboflavin and FAD, in the early stage of the culture and then ATP was produced gradually. Despite many experiments FMN was never detected. On the other hand, formation process of ATP and FAD in the mycelium was also observed, and the conclusion was reached that in the culture of E.ashbyii FAD was produced from riboflavin and ATP.
