2021 Volume 50 Pages 9-15
The nonnative freshwater shrimp Palaemon sinensis (Sollaud, 1911) has been reported at various locations in Japan. This study compared environmental DNA (eDNA) analysis with conventional capturing and investigation as a method to detect P. sinensis in the Tama River. We developed species-specific primers to amplify a 225-bp fragment of the cytochrome c oxidase subunit I gene for eDNA analysis. The invasive shrimps were detected using eDNA analysis in all areas where they were observed using the capture and investigation method. Thus, eDNA analysis can be applied to detect sparsely distributed P. sinensis in the Tama River, making it a useful method to detect invasive shrimps and to help prevent ecological disruption.
