Abstract
Effect of sucrose on the development of freezing tolerance was investigated in suspension-cultured cells of carrot (Daucus carota L.). Noticeable increase in freezing tolerance was observed in the cells precultured on media containing high concentrations of sucrose. The incorporation of sucrose from the medium was suggested from the analyses of sugar and osmotic pressure of cell-free extracts of the precultured cells. Little or no effect of ABA on the development of freezing tolerance was observed when this hormone was added to the media with or without high concentrations of sucrose.
