12. Cryopreservation of Suspension-Cultured Cells of Arabidopsis thaliana by Encapsulation-Dehydration Method(the 46th Annual Meeting)

Abstract

Suspension-cultured cells of Arabidopsis thaliana were encapsulated in alginate gel and cultured in media with high concentrations of sucrose. These cultured cells were cryopreserved in LN_2 after dehydration on silica gel. The encapsulated cells of A. thaliana showed the higher survival rate after dehydration than those of cultured-cells of Oryza sativa and Daucus carota. These results suggest that A. thaliana cells have the high tolerance to dehydration. The cryopreserved cells of A. thaliana sometimes failed to grow and this harmed growth was improved when these cells were precultured on medium with a high concentration of CaCl_2 before culture for regrowing.