Abstract
Noninvasive measurement of concentration using magnetic resonance imaging (MRI) was applied to diffusion of cryoprotectant in aqueous solution, pseudobiological tissues (agar), and biological tissues (liver tissues of chicken). The cryoprotectants were dimethyl sulfoxide and glycerol, common cryoprotectants penetrating cells. ^1H-NMR spectrum, calibration curve of MRI image intensity for cryoprotectant concentration, attenuation of MRI image intensity due to solution fraction and relaxation time in each mass transfer medium were investigated. Transient one-dimensional distribution of cryoprotectant concentration in the medium was measured to estimate apparent diffusivity of cryoprotectant within the medium as a solution in the inverse problem.
