Abstract
Behavior of ice crystals and cells in the biological tissues during the slow-warming after rapid-cooling was visualized microscopically in time-series using a confocal laser scanning microscope with a fluorescent dye. Recrystallization of intracellular ice crystals to increase the mechanical damage of the tissues was investigated quantitatively. Size and number of the ice crystals were measured from the image-data of ice crystals and analyzed statistically to obtain in time-series the frequency, average, and standard deviation of the size of ice crystals, total amount and number density of ice crystals, etc. Influence of the aqueous solution (influence of the additive to the physiological saline) on these characteristics was made clear.
