Abstract
We tested the protective effect of the following reagents on the thermal denaturation of lysozyme: 1)
PvLEA-22, which consists of two tandem repeats of the 11-mer motif characteristic to LEA proteins from an
African sleeping chironomid, 2) its scrambled-type peptide, which has the amino acid composition identical
with that of PvLEA-22, although its sequence is scrambled, and 3) a disaccharide, trehalose. The results of
turbidity, enzyme activity and circular dichroism (CD) spectrum measurements showed that the LEA model
peptides can inhibit the thermal denaturation of lysozyme.
