Effect of Protectants on the Survival Rate of Freeze-dried Lactic Acid Bacteria

Abstract

Effect of lyoprotectants on the survival rate of freeze-dried lactic acid bacteria was investigated. Freeze-drying and preservation of the bacteria with disaccharides (sucrose and trehalose) and bovine serum albumin (BSA) achieved much higher survival rates than that with dextrin. In addidtion, the disaccharide-BSA mixtures showed synergistic effects on stabilization of freeze-dried cells. This result was similar to the stabilization for freeze-dried enzyme. It was suggested that water replacement effect of lyoprotectants was more important than glass transition effect.