Cell Structure and Function
Online ISSN : 1347-3700
Print ISSN : 0386-7196
ISSN-L : 0386-7196

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Human RME-8 Is Involved in Membrane Trafficking through Early Endosomes
Akemi FujibayashiTomohiko TaguchiRyo MisakiMasashi OhtaniNaoshi DohmaeKoji TakioMasashi YamadaJianguo GuMegumi YamakamiMitsunori FukudaSatoshi WaguriYasuo UchiyamaTamotsu YoshimoriKiyotoshi Sekiguchi
著者情報
ジャーナル フリー 早期公開

論文ID: 07045

この記事には本公開記事があります。
詳細
抄録
RME-8 is a DnaJ-domain-containing protein that was first identified in Caenorhabditis elegans as being required for uptake of yolk proteins. RME-8 has also been identified in other species, including flies and mammals, and the phenotypes of their RME-8 mutants suggest the importance of this protein in endocytosis. In the present study, we cloned human RME-8 (hRME-8) and characterized its biochemical properties and functions in endocytic pathways. hRME-8 was found to be a peripheral protein that was tightly associated with the membrane via its N-terminal region. It partially colocalized with several early endosomal markers, but not with late endosomal markers, consistent with observations by immunoelectron microscopy. When cells were transfected with a panel of dominant-active Rab proteins, hRME-8 was confined to large vacuoles induced by expression of Rab5aQ79L, but not by Rab7Q67L. Expression of C-terminally-truncated hRME-8 mutants led to the formation of large puncta and vacuoles, and compromised endocytic pathways through early endosomes, i.e., recycling of transferrin and degradation of epidermal growth factor. Taken together, these results indicate that hRME is primarily involved in membrane trafficking through early endosomes, but not through degradative organelles, such as multivesicular bodies and late endosomes.
著者関連情報
© 2007 by Japan Society for Cell Biology
feedback
Top