Evaluation of the Phagocytosis of Microspheres in V79 Cells by Flow Cytometry

Abstract

Phagocytosis of microspheres in V79 Chinese hamster lung cells was investigated by flow cytometry. Fluorescent microspheres (1.8 μm diameter) were used as the ingesta. Change in the number of V79 cells containing fluorescent microspheres was measured as an index of phagocytic activity. With time there was a sigmoidal increase in cells containing microspheres. The phagocytosis of microspheres is partially explained by two parameters introduced to describe the sigmoidal curves.