抄録
Residual nuclear structures were isolated from Trillium microsporocytes at early meiotic prophase. Relatively well conserved residual nuclear structures were obtained from nuclei isolated in buffer containing 0.1 mM CUSO4. These isolated nuclei were treated with DNaseI, then their chromatin were extracted with 2 M NaCl or dextran sulfate combined with heparin. Cytological and biochemical estimations of the effectiveness of 2 M NaCl and dextran sulfate-heparin on chromatin extraction showed the latter to be the more effective agent. Light microscopy revealed that the residual nuclei retained silver-stainable structures that corresponded to synaptonemal complexes (SCS). The residual nuclei prepared by the treatment with dextran sulfate-heparin consisted mainly of protein that was about 8% of the total nuclear protein present, whereas, about 30% of the nuclear protein was re-tained by nuclei treated with 2 M NaCl.
