Cell Structure and Function
Online ISSN : 1347-3700
Print ISSN : 0386-7196
ISSN-L : 0386-7196
The Minor-Groove Binding DNA-Ligands Netropsin, Distamycin A and Berenil Cause Polyploidisation via Impairment of the G2 Phase of the Cell Cycle
Martin PootKatrin KauschJulia KöhlerThomas HaafHolger Hoehn
著者情報
キーワード: flow cytometry, BrdU hoechst
ジャーナル フリー

1990 年 15 巻 3 号 p. 151-157

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抄録
Distamycin A, netropsin and berenil are knownto cause undercondensation of heterochromatic regions of metaphase chromosomes. These ligands interfere with DNA curvature by binding to the minor groove of the DNA. Whereas the effects of these ligands upon chromatin structure are well established, little is known about their possible interference with cell cycle progression. We show that the presence of these DNA ligands causes protracted cell growth consisting of a prolongation of the Gl phase of the cell cycle along with arrest in the G2 compartment. Concomitant with these cell kinetic disturbances the DNA ligands cause increased polyploidisation. These observations suggest that the DNA-minorgroove may play an important role in progression through the G2phase and proper mitotic transit.
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© Japan Society for Cell Biology
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