Abstract
A cross-linking agent (actinomycin D or mitomycin C) was administered to newborn rats with liver cells actively proliferating in diploid state, to test our recently proposed hypothesis that polyploidization and binucleate formation become manifest, under abortive cell proliferation, in nucleus with cross-links between double strands of DNA. The polyploidization of hepatocyte nuclei was analysed by Feulgen cytofluoromertric method with smear preparations, and binucleate formation was determined on section preparations. The number of polyploid and binuclear cells increased in rat liver treated with a cross-linking agent in comparison with the control. This finding supports our cross-linkage hypothesis.
