Abstract
The relationship was analyzed between cell morphology and steroidogenesis of Y-1 cells to dibutyryl cyclic AMP in dose-related, as well as time sequence responses. Rounded cells were confirmed to be active in steroidogenesis. The time course study showed a bi-phasic pattern of steroid output, i.e., the first phase of 8 h showed a low secretion rate followed by a later accelerated phase for at least 24 h. In stimulant-free medium, most round-up cells showed a return to a flat and polygonal shape within 5 h of incubation. However, the elevated steroidogenic activity persisted for at least 7 h.
