Cell Structure and Function
Online ISSN : 1347-3700
Print ISSN : 0386-7196
ISSN-L : 0386-7196
Growth Conditions on Cytokinesis in Axenic Strains of a Cellular Slime Mold
Masazumi SameshimaTokeo MuroyamaYohichi HashimotoTakuzo Yamada
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ジャーナル フリー

1978 年 3 巻 2 号 p. 123-128

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In axenic strains of Dictyostelium discoideum, approximately one-fourth of vegetative amoebae were multinucleated throughout the growth phase, and synchronous mitosis were observed in these cells. In contrast to vegetative amoebae, all spores of these strains were mononucleated. The percentage of multinucleated amoebae in strain A-3 increased to nearly 70 % when the temperature was raised to 27°C from 22°C, but it decreased during prolonged incubation at 27°C. Karyokinesis proceeded at a constant rate even at 27°C. These results indicate that cytokinesis in this strain is not co-ordinated with karyokinesis. The number of multinucleated cells was drastically reduced in amoebae grown on both live and dead bacteria. Polystyrene latex particles, the diameter of bacteria, did not affect the rate of production.
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© Japan Society for Cell Biology
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