抄録
Simian virus 40 (SV40)-transformed macrophages, clonal line 28-12, and its subline, 28-12(Ara), were used to study morphological changes associated with their growth. Actively growing 28-12 cells were SV40 T-antigen-positive and had thick bundles of microfilaments, a few micro-tubules, and immature nuclei, whereas stationary 28-12 cells had no SV40 T-antigen or a markedly reduced amount of it, and possessed a network pattern of microfilaments similar to that of cultivated macrophages, many microtubules and mature nuclei. 28-12(Ara) cells had a low growth rate, a slightly reduced amount of SV40 T-antigen, a network pattern of microfilaments, significantly fewer microtubules and moderately mature nuclei.
These results suggest that the high growth rate, which is possibly induced by viral products, converts the microfilament distribution from a network to a bundle pattern, the microtubule assembly in the pseudopodia from large to small quantities and the nuclear profile from a mature to an immature one.
