Cell Structure and Function
Online ISSN : 1347-3700
Print ISSN : 0386-7196
ISSN-L : 0386-7196
Preferential Localization of a Subclass of Histone H1 on Nucleosomes Released byDigestion with DNase II
Kei TakahashiYutaka Tashiro
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1981 年 6 巻 4 号 p. 403-407

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Polyacrylamide gel electrophoretic analyses of the nucleosomal histone H1 from rat liver nuclei showed that mononucleosomes and dinucleo-somes, which were rapidly released from rat liver nuclei by digestion with DN-ase II, preferentially contained one subclass of histone H1 (H1B) and lacked another (H1A). In contrast, nucleosomes released by longer digestion had both H1A and H1B. The specific regions of chromatin which contain histone H1B seem to be more susceptibleto DNase II, thus nucleosomes containing histone H1B are released selectively from these regions.
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© Japan Society for Cell Biology
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