Cell Structure and Function
Online ISSN : 1347-3700
Print ISSN : 0386-7196
ISSN-L : 0386-7196
Preparation of Dictyostelium discoideum for Electron Microscopy
Masazumi Sameshima
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1984 Volume 9 Issue 2 Pages 187-192

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Abstract
Dictyostelium discoideum grew and differentiated normally on cellulosedialysis membranes placed on agar plates. This permitted the examina-tion of cells at various stages of development, including cells during aggrega-tion. Cells were preserved well and fixed for electron microscopy by brief ex-posure to acrolein vapore followed by immersion in glutaraldehyde solution.
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© Japan Society for Cell Biology
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