Visualizing Mitochondrial and Plastid Nuclei in Thin Sections of DAPI-stained Cells Using a Confocal 405-nm Laser Scanning Microscope

抄録

Ten-day-old Pelargonium zonale embryos were fixed in formaldehyde and embedded in Technovit 7100 resin. Sections were cut to a thickness of 0.6 μm and stained with 1 μg/ml DAPI. The samples were observed by epifluorescence microscopy using 365-nm excitation and by confocal laser scanning (CLS) microscopy using 405-nm excitation. The mitochondrial and plastid nuclei (nucleoids, DNA and protein complex) were visualized more clearly by CLS microscopy than by traditional fluorescence microscopy.