Karyological Studies on Vigna subterranea (L.) Verdc. from Nigeria

抄録

Cytological investigation was conducted on Vigna subterranean (L.) Verdc. using root squash technique. Apices of root tips pretreated for 3 h with 2 mM 8-hydroxyquinoline, fixed for 24 h with 1 : 3 acetic ethanol (aq.), and hydrolised with 10% HCl were squashed in drops of FLP orcein on clean glass slides. Slides were examined under a Leitx Diaplan research microscope and micrographs of good cells were obtained using Leica WILD MPS microscope camara fitted with a photoautomat. A chromosome number of 2n=22 was recorded for the taxon. The chromosomes varied from metacentric to submetacentric. This is the first report of the karyotype of this species.