Differential Fluorescent Banding Pattern in Three Varieties of Cicer arietinum L. (Fabaceae)

抄録

The karyotypes of 3 varieties in Cicer arietinum L. viz. BS-6, BS-7 and BS-8 were compared after differential staining with orcein, CMA and DAPI. The total chromatin length of BS-6 and BS-8 were 66.20 μm and 61.30 μm whereas 86.00 μm had in BS-7. The centromeric formulae of BS-6 was 12m+4sm, that of BS-7 was 14m+2sm and in BS-8 was 11m+5sm. Six CMA-positive bands were found in BS-6. Variety BS-7 and BS-8 possessed 4 CMA-positive bands in each. 20% GC- rich repeats was found in BS-6. Both BS-7 and BS-8 had 11.70% of GC-rich repeats. One CMA-negative band was found in BS-8 only. Three DAPI- negative bands were found in BS-6 at the same location where the CMA positive bands were present. Reversible banding indicates the presence of GC-rich repeats at those positions. These 3 chromosomes of BS-6 could easily be identified with reversible banding properties. No DAPI-negative band was found in BS-7 and BS-8. One DAPI-positive band was found in 2 chromosomes of BS-8. These chromosomes may consider as DAPI-positive marker. Two satellited chromosomes were found in BS-7 whereas 1 satellited chromosome was found in each BS-6 and BS-8. The satellite of BS-6 showed CMA-positive and DAPI-negative features. The satellites of BS-7 were CMA-positive and slightly DAPI-negative. The satellite of BS-8 showed CMA- and DAPI-positive nature. The different features of the satellites seem to characterize these 3 varieties.