Karyotype Characterization with Fluorescent Banding in One Released and Two Wild Germplasms of Hibiscus cannabinus L.

Abstract

One released (HC-95) and 2 wild (CPI-72126 and KE-1) germplasms of Kenaf (Hibiscus cannabinus L.) were studied to characterize their karyotypes. These germplasms were found to possess 2n=36 chromosomes. Except 2 sub-metacentric chromosomes in CPI-72126 and 1 in HC-95, all the chromosomes of these germplasms were metacentric. No significant difference in the range of chromosomal length and total length of 2n chromosome complements was found among these germplasms. These features revealed that the germplasms have symmetric karyotypes. Four orcein stained satellites were observed in KE-1 and CPI-72126. No satellite was found in CMA and only 2 in DAPI (KE-1). The differential staining property indicated that some satellites were AT-rich and other stain specific. No DAPI band was found in 3 germplasms. However, the number and distribution of CMA bands indicated that each germplasm has distinct CMA-karyotype and thus could easily be characterized.