Cytogenetical and Molecular Characterization of S. violaceum, S. sisymbrifolium and a Putative Hybrid between ♀ S. violaceum and ♂ S. melongena

抄録

Two wild Solanum species, viz. S. violaceum, S. sisymbrifolium, and a putative hybrid between ♀ S. violaceum and ♂ S. melongena were investigated to elucidate their genomic information. For this reason, the orcein and CMA-stained karyotypes of these three specimens were compared. In addition, RAPD with five primer combinations were conducted to make comparative DNA finger printing. The three specimens were found to possess 2n=24 chromosomes. The karyotypes of the three specimens consisted of mostly metacentric chromosomes with no gradual decrease in chromosomal length suggesting symmetric karyotypes. A pair of CMA-positive satellites was found in both the members of pair II of S. violaceum and the putative hybrid. The CMA-karyotype of S. sisymbrifolium was different from the other two specimens. Solanum violaceum and the putative hybrid showed exactly the same DNA finger printing in two primers (Batch-7736-037 and Batch-7736-039) and almost the same in the other three primer combinations. On the other hand, the RAPD finger printing of S. sisymbrifolium was quite different from the other two specimens. The comparative karyotype and RAPD analysis clearly indicated that the putative hybrid was not actually a hybrid between S. violaceum and S. melongena, but rather showed a close resemblance to the mother plant.