Anatomic, Physiologic and Cytogenetic Changes in Allium cepa L. Induced by Diniconazole

抄録

In this study, the possible cytotoxic effects of diniconazol fungicides on root tip cells of Allium cepa L. were investigated. Germination percentage, root length, weight gain, frequency of micronuclei (MN), chromosomal aberrations (CAs) and anatomical changes were used as toxicity markers (indicators). Seeds were divided four groups as one control group treated with tap water, and three treatment groups treated with 25, 50 and 100 ppm doses of diniconazole for 72 h. As a result, germination percentage, root length and weight gain decreased, and MN level and chromosomal aberrations increased in the treatment groups compared to the control group. As a result of microscopic examination, stimulation of the chromosomal damages such as C-mitosis, fragment, chromosome bridge, binucleus cell, abnormal polarization, sticky chromosomes or unequal chromatin distribution by diniconazole treatment were observed. Also, in the root tip cells, diniconazole application caused anatomical damages such as cell deformation, nonspecific vascular tissue, flattening cell nucleus and necrosis.