2017 年 82 巻 1 号 p. 25-31
We report the first chromosome analysis in the Indian vagabond butterflyfish (Chaetodon decussatus) and lined butterflyfish (C. lineolatus) from Andaman Sea, Phuket Province, Thailand. Kidney cell samples were taken from four male and four female fishes. The mitotic chromosome preparation was directly prepared from kidney cells. The chromosomes were stained with conventional and Ag-NOR staining techniques. The results showed that the diploid chromosome number of C. decussatus and C. lineolatus was 2n=48, and the fundamental number (NF) was 48 in both males and females. The chromosomes were present as large telocentric and medium telocentric chromosomes in numbers of 24–24 and 22–26, respectively. There was no observation of strange size chromosomes related to sex. After Ag-NOR banding technique, a single pair of nucleolar organizer regions (NORs) was observed on the long arm centromeric region of medium telocentric chromosome pair 18 in C. decussatus and on the long arm subcentromeric region of medium telocentric chromosome pair 17 in C. lineolatus. The karyotype formulas could be deduced as:
