2017 年 82 巻 4 号 p. 349-354
The present study was undertaken to identify whether any difference exists in karyotype between the tusker (chang Phai) and tuskless (chang Sridor) male Asian elephants (Elephas maximus, Linn. 1758). The elephant using in the present work are owned of some local people who lived in the elephant village, Surin Province, Thailand. Peripheral bloods were collected from the ear-vein of healthy elephants. The lymphocyte culture and harvesting method was employed follow standard protocols. Conventional, GTG-, and Ag-NORs banding techniques were applied to find out the different of both elephant karyotypes. The results showed that the standardized karyotype of both elephants were consisted of one pair of large acrocentric, four pairs of large telocentric, one pair of medium submetacentric, one pair of medium acrocentric, six pairs of medium telocentric, two pairs of small metacentric, two pairs of small submetacentric, and ten pairs of small telocentric homologous chromosomes. The X sex-chromosome was a medium metacentric and the Y sex-chromosome was a small acrocentric chromosome. Here, the NORs bearing chromosome were found on one pair of small submetacentric and two pairs of small telocentric chromosomes similar in both of male elephants. However, those classical cytogenetic techniques could not be distinguished the karyotype of tusker and tuskless male elephants. At least, we present the standardized karyotype, idiogram and the basic cytogenetic data that could be served as a basis for future chromosome analyses of Asian elephant populations and for comparative cytogenetic studies with other related species. The karyotype formula could be deduced as:
