Abstract
The eggs of the carp (Cypriiius carpio), during about 5 to 10 minutes after insemination, were exposed to low temperatures between 0.5° and 3°C for about 10 to 30 minutes.
Refrigeration prevented the second maturation division from going to completion and caused the chromosome duplication in the egg due to a retention of the haploid set of chromosomes normally going into the second polar body, leading to the production of the diploid egg nucleus.
It is highly probable that union of the diploid egg nucleus thus induced with the sperm nucleus which is haploid, would give rise to triploid zygotes and consequently to triploid individuals.
