CYTOLOGIA
Online ISSN : 1348-7019
Print ISSN : 0011-4545
A Cytochemical Study of Nucleic Acids in Plant Cells
III. Effects of proteins on Feulgen reaction in vitro
Masahiro Ruè Ishida
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ジャーナル フリー

1959 年 24 巻 1 号 p. 107-114

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抄録
1. Effects of proteins on the Feulgen reaction in vitro were examined.
2. The absorption maxima of colors developed with Na·DNA-protein mixtures were determined. The results of the experiment show that thymus histone and sperm protamine shift the maximum towards the longer wavelength whereas gelatin, casein and albumin do not shift.
3. Progression of the color development against time in the presence of histone·sulfate at pH 1.16, 2.28 and 3.68 was compared with those of Na·DNA alone.
4. Relation between concentrations of the proteins contained in DNA solution and color intensities was investigated.
5. Effects of pH and proteins on the color intensities were investigated.
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© The Japan Mendel Society
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