Abstract
A type of spiral growth has been found in isolated single cells of the gametophytic callus tissue of Pteris vittata. Spiralization occurred by the twisting of cell wall with a regular interval and unequal elongation in both sides of the cell. This is the first report on spiral growth found in isolated single cells of higher plants. The callus cells have often been connected by thin membranes or membranous fragments. This can be regarded as an evidence for the presence of files of daughter cells surrounded by the stretched wall of the original parent cell in living material.