Induction of Mitotic Abnormalities in Onion Root-Tips by Tobacco Smoke Condensate
1973 年 38 巻 4 号 p. 707-712
詳細
1973 年 38 巻 4 号 p. 707-712
Onion root-tips were used to study the effects of water-soluble extract of tobacco smoke condensate (TSC) on various cytological parameters. When roots were treated with different concentrations of TSC (0.04%, 0.1%, 0.05% and 0.25%) a number of abnormalities resulted. These included vacuolization of the cytoplasm as well as abnormal mitotic stages. The abnormalities consisted of scattering of chromosomes during metaphase, and bridging during anaphase. Also with in-creasing concentrations of tobacco smoke condensate, the mitotic index declined. This investigation should be of importance in assessing the biological effects of tobacco smoke and its constituents.
