Feulgen Type Staining of Deoxyribonucleic Acid with Janus colours

抄録

The use of janus black, janus blue and janus red as aqueous solutions in the staining of acid hydrolysed tissue sections is recommended. Janus black-SO₂, when prepared with N HCl and potassium metabisulphite and used in the staining of acid hydrolysed DNA reveal well stained nuclei at pHs 2.0, 3.0 and 4.0. Sections stained with janus blue-SO₂ show optimum staining at pHs 3.0 and 4.0. Janus red-SO₂ prepared by this method reveal very pale staining of the nuclei. Janus black and janus blue when prepared with sodium hydrosulphite and used in the staining of DNA following the Feulgen procedure reveal nuclei which are of magenta colour. Janus black, charged with sodium hydrosulphite, becomes metachromatic in staining nuclei with a magenta colour and blue mucus glands as studied with rat rectum. Unhydrolysed sections stained with these dyes also show well stained nuclei and the cytoplasm and alkali hydrolysed sections stained with them show only nuclei. These phenomena indicate that staining of both DNAP and RNAP and also DNAP alone is possible with these dyes. Staining with janus red without any primary anino group in its molecule has been considered as modified Feulgen reaction and that with janus black and janus blue with primary amino group in their molecules as due to Feulgen type of reaction. The absorption characteristics of these dye solutions as well as those of nuclei stained with these dyes are presented in this paper.