Short Pulse Cytochalasin B Treatment of Mouse Fibroblasts

抄録

Mouse L-cells in log phase were treated with cytochalasin B for time periods ranging from 20 sec to 1 h and subsequently examined by means of scanning electron microscopy. Zeiotic blebs appeared within the shortest time period in all cells but was accompanied by a general loss of microvilli in spindle and stellate shaped cells. It is suggested that initial responses in zeiotic bleb formation may be dictated by the amount of pre-existing membrane per unit area of the cell surface, i. e., the extensiveness of microvilli.