Abstract
With the coverglass culture method, a relation between the temperature and the growth of the fibroblast in vitro was investigated. The growth was measured by tracing the surface increase.
1) The growth of the tissue, which had been kept at 39° for one day after the preparation, was not a rectilinear function of the temperature between 25° and 43°, the maximum of the growth being at 39° and the minimum at 25°
The growth between 25° and 39° could be approximately expressed with 2 exponential lines corresponding much more to VAN'T HOFF-ARRHENiUS's formula than to VAN'T HOFF's, with the point of inter-section lying at 30°.
2) With culture tissue, which had been kept at 39° for 5 hours after the preparation the temperature influence an the growth was found to be not seriously different from the result in the former Gase. The temperature coeflicient Q10, was generally much larger than that of VAN'T HOFF's law, especially extraordinarily large between 25° and 27°. So also was the value of μ of VAN'T HOFF-ARRHENIUS.
3) The growth of the tissue under alternating temperatures, but essentially under the saure temperature, that is, the growth of the tissue, which, after having been incubated at 39° for 5 hours after the preparation, was kept at a higher temperature an the first day and at a lower temperature an the second day, or vice versa, showed no essential differente between 30° and 39°. But there was a distinct differente in the Gase of 25° and 30°. The tissue which was kept first at 25° and then at 30° showed a smaller growth in comparision with that which was kept first at 30° and then at 25°.
