Cytogenetical Studies on the Effect of Some Chlorinated Pesticides
I. Effect on somatic chromosomes of Lens and Pisum
1987 Volume 52 Issue 1 Pages 47-53
Details
1987 Volume 52 Issue 1 Pages 47-53
Lens esculenta, L.culinaris, Pisum sativum and P. arvense which were included in the present study belong to one of the economically imporatnt families-Leguminosae. The somatic chromosomal anomalies were studied after root-tip (1/2, 1 and 2 hr. treatment) and seed-soak (24 hr. treatment) followed by fixation in Farme's fixative. Root-tip squashes were made in 1% acetocarmine. The used concentrations of Benzene hexachloride (BHC), Lindane and Endrin were 0.01%, 0.02%, 0.03% and 0.04% and of Aldrin and Heptachlor were 0.05%, 0.1%, 0.2% and 0.3%. A considerable fall in mitotic index was noticed from control in all treatments. The most common chromosomal abnormalities induced by these pesticides were-inactivation of spindle mechanism, condensation of chromosomes below their normal size, chromatin bridges at anaphase and telophase. Multipolar spindles were observed after BHC, Lindane and Endrin treatments. Only BHC and Lindane were found to induce C-mitosis. However, Lindane was found to be a less potent polyploidizing agent. Multinucleate cells were observed only in BHC treatments. Fragments were observed occassionally in BHC, Lindane, Endrin and Heptachlor treatments.
