The Complementary Restoration of Plant Regeneration- and Flower Initiation-ability Through Somatic Cell Fusion

抄録

Protoplasts from line Tx4 (Nicotiana tabacum), a p-fluorophenyl alanine (PFP) resistant cell line that has lost the ability to regenerate whole plants, were fused using dextran with leaf protoplasts of DR (N. tabacum), a 2, 4-dichlorophenoxyacetic acid resistant and plant regenerable line that has lost flower initiation ability. Somatic hybrids were selected initially by culture in PFP levels which inhibit the growth of DR and then for plant regeneration. The plants suspected to be somatic hybrids were regenerated from two colonies (H-l, H-2) and formed flower buds within six months, while DR plants never formed even if grafted on normal plants. Callus induced from H-1 and H-2 showed resistance to both PFP and 2. 4-D with the resistance being expressed as semidominant characters. The results indicate drug resistance to PFP and 2, 4-D and morphogenic ability for plant regeneration and flower initiation were restored complementally through somatic hybridization.