Anther-culture-derived 2x-Plants in Cabbage (Brassica oleracea L. ssp. capitata L.)
I. Meiotic abnormalities
1989 年 54 巻 3 号 p. 597-603
詳細
1989 年 54 巻 3 号 p. 597-603
Of 29 anther-culture-derived 2x-plants of cabbage (Brassica oleracea L. ssp. capitata L.), 45% were male sterile (ACMS) and 55% male fertile (ACMF). Chi-square test was used to compare the percentage of pollen mother cells (PMCs) with meiotic abnormalities in ACMF or ACMS plants with those in anther donor (control) plants. In metaphase I, univalents, trivalents and quadrivalents were frequently observed but only in the ACMS group were these abnormalities significantly higher than in the control. In anaphase I to telophase I, there were significantly higher occurences of laggards and other abnormalities including unequal chromosome segregation in both ACMF and ACMS groups; there were 2.6 time more laggards in the ACMS group. In anaphase II to telophase II, micronuclei, tripoles, unequal chromosome distribution and sporads with dyads and triads were frequently observed in both ACMF and ACMS but, in all cases, occurrences were markedly higher in the ACMS group accompanied by significantly lower 9-9-9-9 equal chromosome segregation. High incidence of sterility and chromosomal abnormalities in anther-culture-derived 2x-plants, particularly in the ACMS group, appears to be the result of homozygosity induced by anther culture.
